In Vitro Maturation of Goat Oocyte

DIFFERENT DOSE OF FETAL CALF SERUM SUPPLEMENTATION ON THE MEDIUM TO INCREASE IN VITRO MATURATION

ABILITY OF GOAT OOCYTE

DEVITA HERY FATMAWATI

06/194971/PT/05134

ABSTRACT

            The research was conducted to evaluate the effect of Fetal Calf Serum (FCS) as protein supplements for in vitro maturation. Goat ovaries were obtained from a local slaughterhouse and transported to the laboratory in a flask of saline (0.9% NaCl) at temperature 31-34^oC. Follicles of 1-6 mm in diameter were aspirated with an 23G needle and syringe 3 ml. Searching of oocytes were done under stereo microscope. Oocytes were washed 3 times with Dulbecco’s Phosphate Buffered Saline (DPBS) and Tissue Culture Medium-199 (TCM-199) then matured into drop of medium in disposable tissue dish under 38-39^oC, 95% humidity, and CO₂ 5% for 24 hours. Oocytes were divided into three groups based on the dose: a. V/0 (goat oocytes without FCS supplementation); b. V/10 (goat oocytes with 10% FCS supplementation); c. V/20 (goat oocytes with 20% FCS supplementation). Data was analyzed by using oneway ANOVA with the dose of FCS as the main factor. Percentages of mature oocytes were reported descriptively. Result of the present study showed dose of FCS had no significant effect on in vitro maturation ability. The percentages of mature oocytes from three groups were 61.61±7.13%; 84.90±4.25%; and 93.26±3.25%, and the percentages of damaged oocytes were 24.40±7.85%; 10.16±3.59%; and 1.25±1.25% for 0% FCS, 10% FCS, and 20% FCS, respectively. Based on the result in could be concluded that the ability of in vitro maturation of goat oocytes was high (>50%), with biggest percentage got from oocytes that supplemented with 20% FCS.

(Key words: Fetal Calf Serum, Local goat oocytes, In vitro maturation, Percentage of mature oocytes)